|
| Antibodies |
|
| Rabbit anti-Abca12 |
Zuo et al.35
|
N/A |
| Goat anti-Ctsd |
R&D Systems |
Cat # AF1029 |
| Chicken anti-GFP |
Abcam |
Cat # ab13970 |
| Rabbit anti-Gjb2 |
Fisher Scientific |
Cat # 71–0500 |
| Rabbit anti-GlcCer |
Glycobiotech |
Cat # RAS_0011 |
| Rabbit anti-K10 |
Covance |
Cat # PRB-159P |
| Chicken anti-K14 |
Biolegend |
Cat # 906004 |
| Goat anti-K14 |
Santa Cruz |
Cat # sc-17104 |
| Rabbit anti-K14 |
Covance |
Cat # PRB-155P |
| Goat anti-K79 |
Santa Cruz |
Cat # sc-243156 |
| Rabbit anti-K79 |
Abcam |
Cat # ab7195 (Ag7195)27
|
| Rabbit anti-Ki67 |
Cell Signaling |
Cat # 12202S |
| Goat anti-Klk6 |
R&D Systems |
Cat # AF2008 |
| Goat anti-Klk7 |
R&D Systems |
Cat # AF2624 |
| Rabbit anti-Rab11a |
Cell Signaling |
Cat # 2413 |
| Rabbit anti-S100a9 |
Cell Signaling |
Cat # 73425 |
| Rabbit anti-Sprr2d |
Proteintech |
Cat # 23046–1-AP |
| InVivoMAb anti-mouse IL-17A |
BioXCell |
Cat # BE0173, clone 17F3 |
| InVivoMAb mouse IgG1 isotype control |
BioXCell |
Cat # BE0083, clone MOPC-21 |
|
| Biological samples |
|
| Mouse tissue samples, obtained in accordance with guidelines established by the University of Michigan Unit for Laboratory Animal Medicine |
This manuscript |
Study protocol # PRO00011782 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Acetone |
Sigma |
Cat # 650501 |
| Cacodylate solution |
Electron Microscopy Sciences |
Cat # 11652 |
| Calcium chloride solution |
Electron Microscopy Sciences |
Cat # 12340 |
| Corn oil |
Sigma |
Cat #C8267 |
| Glutaraldehyde solution |
Electron Microscopy Sciences |
Cat # 16300 |
| InVivoPure pH 7.0 Dilution Buffer |
BioXCell |
Cat # IP0070 |
| Paraformaldehyde solution |
Electron Microscopy Sciences |
Cat # 15700 |
| Potassium ferricyanide |
Sigma |
Cat # 702587 |
| Potassium ferrocyanide |
Sigma |
Cat #P9387 |
| Tamoxifen |
Sigma |
Cat #T5648 |
| X-Gal |
Roche |
Cat # 10651745001 |
|
| Critical commercial assays |
|
| RNAscope 2.5 HD Reagent Kit-BROWN |
ACD |
Cat #322310 |
| RNAscope 2.5 Pretreat Reagents-H202 and Protease Plus |
ACD |
Cat # 322330 |
| RNAscope Target Retrieval |
ACD |
Cat # 322000 |
| RNAscope Wash Buffer |
ACD |
Cat #310091 |
| RNeasy Mini Kit |
Qiagen |
Cat # 74104 |
|
| Deposited data |
|
| Data for RNA sequencing of KA skin |
This study |
GEO: GSE254889
|
| Data for E17.5 Abca12 mutant mouse embryos |
Cottle et al.45
|
GEO: GSE56125
|
| Data for human HI patients |
Enjalbert et al.44
|
GEO: GSE131903
|
|
| Experimental models: Organisms/strains |
|
| Mouse: Abca12tm1a (KO or “LacZ” allele) |
EUCOMM |
Clone # HEPD0708–3-F11 |
| Mouse: Abca12tm1c (cKO or “c” allele) |
This study |
N/A |
| Mouse: Lrig1tm1.1(cre/ERT2)Rjc (Lrig1-CreERT2) |
The Jackson Laboratory42
|
Cat # 018418 |
| Mouse: Tg(KRT5-cre)5132Jlj (K5-Cre) |
Ramirez et al.38
|
MGI:3050065 |
| Mouse: Tg(KRT14-cre/ERT)20Efu/J (K14-CreERT) |
The Jackson Laboratory81
|
Cat # 005107 |
| Mouse: Krt79-Cre (K79-Cre) |
Mesler et al.48
|
N/A |
| Mouse: Shhtm1(EGFP/cre)Cjt/J (Shh-Cre) |
The Jackson Laboratory82
|
Cat # 005622 |
| Mouse: Gt(ROSA)26Sortm1(EYFP)Cos (YFP reporter) |
The Jackson Laboratory83
|
Cat # 006148 |
| Mouse: B6J;B6N-Tg(CAG-Flpo)1Afst/Mmucd
|
Kranz et al.84
|
MGI:4453967 |
| Mouse: C57BL/6J
|
The Jackson Laboratory |
Cat # 000664 |
| Mouse: NU/J
|
The Jackson Laboratory |
Cat # 002019 |
|
| Oligonucleotides |
|
| Genotyping primer WT-1F: 5'-GCTCTCTCTCTCTCTTCCTCTTC-3' |
This manuscript |
N/A |
| Genotyping primer CSD-F: 5' -CACACCTCCCCCTGAACCTGAAAC-3' |
This manuscript |
N/A |
| Genotyping primer 3R: 5'-GAACTTACTTGAATAAAGCATTGCAGGC-3' |
This manuscript |
N/A |
|
In situ probe: Mouse IL-17a |
ACD (RNAscope) |
Cat # 319571 (Mm-Il17a) |
|
| Software and algorithms |
|
| Adobe Photoshop CS6 |
Adobe Inc. |
https://www.adobe.com
|
| AxioVision, Version 4.8.2 |
Carl Zeiss |
https://www.zeiss.com/microscopy/us/products/microscope-software/axiovision.html
|
| CK Multi-Probe software, Version 2.2.0.1/3/22/2016 |
Courage + Khazaka GmbH |
https://www.courage-khazaka.de/en/
|
| clusterProfiler |
Yu et al.85
|
https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
|
| DESeq2 |
Love et al.86
|
https://bioconductor.org/packages/release/bioc/html/DESeq2.html
|
| Prism, Version 6 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/
|
|
| Other |
|
| Cloning cylinders |
Bel-Art |
Cat # 378470200 |
| Tewameter |
Courage + Khazaka GmbH |
Cat # TM300-MPA5 |
