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. 2024 Aug 12;22:754. doi: 10.1186/s12967-024-05512-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — Clinicopathological characteristics of organoid lines (A) A thickened gastric antrum wall is shown by CT scanning. The red box marks the enlarged image. (B) An ulcerated mass is observed at the antrum in the gastrectomy sample with the enlarged lymph node near the right gastroepiploic artery (T, primary tumor; LN, lymph node). (C) From left to right, histology of poorly-differentiated adenocarcinoma of original tissues by H&E staining, organoid morphology by light- field observation, and organoid morphology by H&E staining. The histology is compatible with poorly-differentiated adenocarcinoma with irregular lumens. The upper panel indicates SPDO1P and the lower panel indicates SPDO1LM. Scale bar, 100 μm. (D) Immunohistochemical staining of E-cadherin, N-cadherin, and Ki67 for SPDO1P and SPDO1LM. Scale bar, 80 μm. (E) SPDO1P and SPDO1LM maintain cell vitality in continuous culture in vitro. Both organoid lines expanded for over 50 generations. Note: P1 = passage 1, P15 = passage 15, and P50 = passage 50. Scale bar, 100 μm