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. 2024 Aug 12;22:754. doi: 10.1186/s12967-024-05512-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — Culture conditions of SPDO1P and SPDO1LM. (A) The SPDO1P and SPDO1LM lines grow well in complete medium in 3D culture (light field). Scale bar, 100 μm. (B) The growth curves of SPDO1P (red) and SPDO1LM (blue) in 3D culture show that the doubling time of SPDO1P (120 h) is longer than that of SPDO1LM (72 h). (C) The SPDO1LM cells can adhere to the wall in 2D culture with 10% FBS-DMEM medium (light field). Scale bar, 100 μm. (D) The growth curve of SPDO1LM in 2D culture with a doubling time of 96 h. (E) Comparison of cell vitality of the SPDO1LM line based on the stepwise elimination of growth factors or chemicals. (F) Comparison of cell vitality of the SPDO1P line based on stepwise elimination of growth factors or chemicals. (G) The growth of SPDO1P (upper panel) and SPDO1LM (lower panel) cultured in complete medium (left), without Y27632 (middle), and 10% FBS-DMEM (right) under light-field observation. The SPDO1LM line grows well in complete medium, without Y27632, and 10% FBS-DMEM, while the SPDO1P line shows lower cell vitality without Y27632 or 10% FBS-DMEM. Scale bar, 200 μm, ^nsP > 0.05, ^*P < 0.05, ^***P < 0.001. Abbreviation: DMEM, Dulbecco’s modified Eagle medium; FBS, fetal bovine serum