Fig. 3.

STRs and karyotypes analysis, as well as tumorigenesis of SPDO1P and SPDO1LM organoid lines. (A) The crucial STRs loci are compared between SPDO1P and SPDO1LM lines. Both organoid lines show XX sex alleles. STRs loci are matched well at D5S818, vWA, D7S820, TH01, D13S317, and TPOXSTR. There is allele drift at the loci of D16S539 and CSF1PO. (B) The chromatograms of the D5S818 locus (red) and D16S539 locus (green) from SPDO1P and SPDO1LM lines. The D5S818 locus is matched well, and one allele is shifted at the D16S539 locus. (C) The karyotype of SPDO1P line is 46, XX, + 8, -21 (upper), while that of the SPDO1LM line is 48, XX, + 8, +12, r(21)(p13q22.3) (lower). (D) The tumor volume of the SPDO1LM xenograft is larger than that of the SPDO1P xenograft (385.76 ± 112.16 vs. 63.46 ± 10.55 mm³). (E) By H&E staining, both xenografts of SPDO1P and SPDO1LM are poorly-differentiated adenocarcinoma. Scale bar, 100 μm.;^**P < 0.01