Table 1.
Biological characteristics of SPDO1P and SPDO1LM organoid lines
| Parameters | Characteristics | SPDO1P (Primary tumor) |
SPDO1LM (Lymph node) |
|---|---|---|---|
| Growth | Culture time | 570 days | 570 days |
| Doubling time | 120 h (3D) | 72(3D) or 96 (2D) hours | |
| 3D culture | Yes | Yes | |
| 2D culture | No | Yes | |
| Culture medium |
Essential cytokine (Noggin, Y27632, SB202109, EGF, R-Spondin1) WNT3a-free |
Essential cytokine or 10% FBS DMEM WNT3a-free |
|
| STR | Unique | Unique | |
| Karyotype | 46, XX, + 8, -21 | 48, XX, + 8, +12, r(21)(p13q22.3) | |
| Xenograft | 100% | 100% | |
| Genomics | CNV | 8 gain, 21 loss | 8 gain, 12 gain, 21 loss |
| TP53 | Wild-type | Wild-type | |
|
Mutation (shared) |
CIC, SETD1B, LAMA2, IL21R, FAT4, KAT6B, ERBB3, CEP89, LRP1B, DLG1, MUC16, FGFR2, MAGI2, SVEP1, SPEG, EPHA1, PAX3, PREX2, PTPRT, ZNF132, CACNA1D, PRPF8, SYNE1, LRRK2, CTCF | CIC, SETD1B, LAMA2, IL21R, FAT4, KAT6B, ERBB3, CEP89, LRP1B, DLG1, MUC16, FGFR2, MAGI2, SVEP1, SPEG, EPHA1, PAX3, PREX2, PTPRT, ZNF132, CACNA1D, PRPF8, SYNE1, LRRK2, CTCF | |
|
Mutation (special) |
KRAS, PIK3C2B, BRAF, AKT1, FAT1, CLASP2 | EPHA7, FAT3, EPPK1, FLNA, CDKN1B, KMT2B | |
| ScRNA-Seq | Up-regulated pathway | Oxidative phosphorylation, MYC targets V1 | Angiogenesis, epithelial-mesenchymal transition, glycolysis, hypoxia |
| Behaviors | Migration ability | Weak | Strong |
| Invasion ability | Weak | Strong | |
| Angiogenesis | Weak | Strong | |
| Drug sensitivity | 5-Fu (IC50) | 0.4125 μm | 0.1267 μm |
| OXA (IC50) | 2.7940 μm | 5.6873 μm | |
| PTX (IC50) | 1.7040 nm | 1.8463 nm |