Fig. 2.

Viral load of PCV3 in culture supernatants quantified via real-time PCR. Serial passaging of PCV3 in primary porcine bone marrow cells; the generations 1–5 were cultured for 9, 22, 31, 30, and 37 days, respectively. Viral replication in infected culture supernatants was measured using real-time PCR. Real-time PCR data are expressed as log10 genomic copies per 1 mL supernatant