FIG. 1.
In vitro binding of Mo-MuLV Gag to Nuc(212). An extract of Mo-MuLV-infected NIH 3T3 cells was divided and mixed with extracts of bacteria expressing either GST or GST-Nuc(212); the GST proteins were recovered on glutathione-coated beads. Proteins in whole lysates and proteins bound to the beads and eluted were analyzed by Western blotting using a polyclonal antibody against capsid protein. Migration of size marker is shown on the right. Positions of Pr65gag and capsid are indicated on the left.