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Mo-MuLV harboring the L477P mutation fails to assemble. 293T cells were transfected with 10 μg of plasmid expressing either Mo-MuLV or L477P mutant. Mock, transfection without adding plasmid DNA. Cell extracts (bottom) and purified virions (top) were analyzed by Western blotting as described for Fig. 2A. The membrane was probed with polyclonal anticapsid serum. Positions of migration of size marker bands (right) and of full-length Gag (Pr65gag) and capsid (left) are shown.
