Fig. 5. Time-dependent COX-2 protein expression post UVR treatment in HaCaT keratinocytes and 46BR.1N fibroblasts (A) and (B) and in silico prediction (C) and (D). Normalised mean protein expression (n = 3 independent experiments) and representative western blots for COX-2 and GAPDH (loading control) in HaCaT keratinocytes (A) and 46BR.1N fibroblasts (B) before (basal) and post UVR treatment (15 mJ cm⁻² UVR). Data expressed as mean ± SEM. “HaCaT + UVR” (C) and “46BR.1N + UVR” (D) in silico experiments, showing median COX-2 protein concentration over 6 h in 1000 model variants.