FIG. 2.
Nef binds Pak2 in human T cells and monocytic cells. (A) Western blot analysis for NefSF2 expression in the human T-cell lines CEM, HuT-78, and Jurkat. (B) In vitro kinase assay followed by caspase 3 cleavage of anti-Nef immunoprecipitates from cell lysates obtained from each cell line. In all three cases the Nak immunoprecipitated was sensitive to caspase 3 treatment, and this cleavage was inhibited by ZVAD. (C) Western blot analysis for NefSF2 expression in two human monocytic cell lines, U937 and THP-1. (D) In vitro kinase assay followed by caspase 3 treatment of Nef immunoprecipitates from cell lysates obtained from each cell line. Nak was found to be caspase 3 sensitive in both monocytic cell lines.