Table 1.
Primer set | Sense 5'-- 3' | Antisense 5'--- 3' | Size | Annealing |
p16 M | TTATTAGAGGGTGGGGCGGATCGC | GACCCCGAACCGCGACCGTAA | 150 | 62°C |
p16 U | TTATTAGAGGGTGGGGTGGATTGT | CAACCCCAAACCACAACCATAA | 151 | 62°C |
RARβM | TCGAGAACGCGAGCGATTCG | GACCAATCCAACCGAAACGA | 146 | 55.5°C |
RARβU | TTGAGAATGTGAGTGATTTGA | AACCAATCCAACCAAAACAA | 146 | 55.5°C |
MGMT M | TTTCGACGTTCGTAGGTTTTCGC | AACCAATCCAACCAAAACAA | 81 | 55.7°C |
MGMT U | TTTGTGTTTTGATGTTTGTAGGTTT | AACTCCACACTCTTCCAAAAAC | 93 | 55.7°C |
FHIT M | TTGGGGCGCGGGTTTGGGTTTTTA | CGTAAACGACGCCGACCCCACTA | 74 | 55.5°C |
FHIT U | TTGGGGTGTGGGTTTGGGTTTTTA | CATAAACAACACCAACCCCACTA | 74 | 55.5°C |
DAPK M | GGATAGTCGGATCGAGTTAACGT | CCCTCCCAAACGCCGA | 98 | 55.7°C |
DAPK U | GGAGGATAGTTGGATTGAGTTAAT | CAAATCCCTCCCAAACACCAA | 106 | 55.7°C |
APC M | TATTGCGGAGTGCGGGTC | TCGACGAACTCCCGACGA | 100 | 56°C |
APC U | GTGTTTTATTGTGGAGTGTGGGTT | CCAATCAACAAACTCCCAACAA | 110 | 56°C |
GSTp1 M | TTCGGGGTGTAGCGGTGGTC | GCCCCAATACTAAATCACGACG | 91 | 56°C |
GSTp1 U | GATGTTTGGGGTGTAGTGGTTGTT | CCACCCCAATACTAAATCACAACA | 97 | 56°C |
H19 M | TTATAAAATCGAAAATTACGCGCGA | AGATGATTTTCGTGAATTTTGCG | 136 | 55°C |
H19 U | TATAATTATAAAATCAAAAATTACA | TTTTAGATGATTTTTGTGAATTTT | 145 | 55°C |
ER M | CGGTTGGAGTTTTTGAATCGTTC | CTAGCGTTAACGACGACCG | 151 | 55°C |
ER U | ATGAGTTGGAGTTTTTGAATTGTTT | ATAAACCTACACATTAACAACAACCA | 158 | 55°C |
Clone 1.2 M | ATGAGTTGGAGTTTTTGAATTGTTT | AATAATAAACGTAACGCCCGCGAAC | 258 | 63°C |
Clone 1.2 U | GGTTGTTTGGTTTTTATTGGGATGTTTTT | CCTAAATAATAAACATAACACCCACAAAC | 258 | 63°C |