FIG. 2.

Northern analyses of RNA fractions isolated from cells that were mock infected (lanes 1 and 5) or infected with the wild-type virus (WT; lanes 2 and 6), RvM37 (lanes 3 and 7), and RqM37 (lanes 4 and 8). NIH 3T3 cells (10⁷) were infected with each virus at an MOI of 5 PFU per cell and harvested at 24 h postinfection. RNA samples (20 to 30 μg) were separated on agarose gels that contained formaldehyde, transferred to a nitrocellulose membrane, and hybridized to a ³²P-radiolabeled probe that contained the sequence of M37 (lanes 1 to 4) or M25 (lanes 5 to 8). Sizes are indicated in kilobase pairs.