Table 3.
Annotation correction of ORF translational start position
| ORF | Gene | Unitag position (occurrence) | Chr | New ATG position | Predicteda |
|---|---|---|---|---|---|
| YDL208W | NHP2 | 6(1) 28(2) 48(1) | 4 | +51 | Yes |
| YDR378C | LSM6 | 88(2) 107(1) | 4 | +111 | No |
| YER030W | 0(3) 8(1) 12(1) 20(2) 404(1) | 5 | +21 | Yes | |
| YER050C | RSM18 | 133(2) 297(1) 571(1) | 5 | +192 | No |
| YGR088W | CTT1 | 8(2) 377(1) | 7 | +33 | No |
| YHR163W | SOL3 | 91(2) 848(1) | 8 | +93 | Yes |
| YIL043C | CBR1 | 82(1) 94(1) 102(3) | 9 | +114 | Yes |
| YIL053W | RHR2 | 35(5) 36(1) 349(1) 526(1) 852(1) | 9 | +63 | No |
| YIL076W | SEC28 | 177(4) 556(1) | 9 | +189 | No |
| YJL046W | 99(2) | 10 | +126 | No | |
| YKR042W | UTH1 | 48(3) 57(2) 413(1) 859(1) | 11 | +255 | Yes |
| YOR091W | 96(1) 106(2) | 15 | +168 | No | |
| YOR147W | MDM32 | 56(3) | 15 | +93 | No |
| YPR169W | JIP5 | 32(1) 50(1) 55(2) | 16 | +66 | Yes |
Proposed translational start codon changes for 14 genes based on 5′ SAGE TSS data and multiple orthologous sequences alignment. 5′ SAGE TSS position are shown relative to the currently annotated ATG, with unitag occurrence in parenthesis and multiple occurrence unitags are shown in bold. The proposed new downstream ATG start codon position relative to the original start position is listed.
a‘Predicted’ means the annotation change had been previously suggested by multiple Saccharomyces species comparison (42).