FIG. 1.
HCV NS5B can protect RNA from digestion by RNase A. (A) Sequence and secondary structure of SLC1 (20). (B) Autoradiogram of a 20% denaturing gel containing products protected from RNase A digestion by NS5B. SLC1, a 35-nt RNA (derived from nts 2039 to 2069 of plus-strand BMV RNA3), was labeled during transcription with 32P-CMP. The RNA products of the BMV replicase from proscript −20/13 are of 13 and 14 nts (34), and their positions are indicated to the left of the autoradiogram. RNase A (1 μl of a 0.2-μg/μl solution) and HCV NS5B additions are indicated above the autoradiogram.