FIG. 8.

Requirements in the 3′ single-stranded (SS) tail of SLD3 for RNA synthesis. (A) Sequences of the 3′ tail in SLD3 and in the various mutants. The sequences of the stem 5′ of the tail are not changed from those in SLD3 and are not shown. Deletions are denoted by dashes, and nucleotide substitutions and insertions are denoted by bold lowercase letters. (B) Autoradiogram of the products synthesized from SLD3 and the various mutants shown in panel A. Φ denotes a reaction performed in the absence of an exogenous template. The white asterisk shows the position of the monomeric (25-nt) RdRp product.