Table 2.
Accuracy and precision of PAH8 using two different extraction methods
| Compounds | Nominal concentration (µg/kg) | Alkali digestion method | Ultrasonication method | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Intra-daya (n = 5) | Inter-dayb (n = 3) | Intra-day (n = 5) | Inter-day (n = 3) | ||||||
| Accuracy | Precision | Accuracy | Precision | Accuracy | Precision | Accuracy | Precision | ||
| (%) | (% RSDc) | (%) | (% RSD) | (%) | (% RSD) | (%) | (% RSD) | ||
| B[a]A | 5 | 97.35–100.00 | 1.92–3.42 | 99.74 | 1.09 | 98.43–101.39 | 0.53–2.27 | 100.70 | 0.84 |
| 10 | 98.80–101.02 | 1.07–3.41 | 99.79 | 0.62 | 99.54–103.56 | 1.87–2.17 | 100.45 | 0.80 | |
| 20 | 99.98–106.03 | 1.87–4.08 | 102.13 | 2.28 | 102.18–106.83 | 1.57–2.43 | 102.11 | 1.50 | |
| Chry | 5 | 98.30–100.27 | 0.76–0.99 | 99.85 | 0.68 | 98.38–100.72 | 1.05–2.26 | 99.77 | 0.53 |
| 10 | 96.75–99.73 | 1.72–3.13 | 99.52 | 0.81 | 98.29–100.83 | 0.82–2.21 | 99.93 | 0.26 | |
| 20 | 99.50–106.62 | 0.93–7.42 | 100.01 | 0.24 | 102.41–103.05 | 0.80–3.71 | 100.77 | 3.32 | |
| B[b]F | 5 | 97.92–98.47 | 1.20–2.59 | 99.93 | 0.47 | 96.82–98.15 | 0.55–1.20 | 98.60 | 0.66 |
| 10 | 97.23–97.76 | 2.66–4.86 | 99.58 | 0.42 | 97.78–98.06 | 0.60–0.76 | 98.65 | 0.10 | |
| 20 | 98.24–105.74 | 1.12–8.35 | 100.15 | 0.08 | 98.35–98.92 | 0.39–0.57 | 99.20 | 0.31 | |
| B[k]F | 5 | 99.07–100.30 | 0.87–2.49 | 99.99 | 0.07 | 96.29–100.39 | 1.32–4.22 | 98.82 | 0.60 |
| 10 | 98.49–99.64 | 2.32–3.22 | 100.24 | 0.13 | 89.93–96.94 | 2.83–6.72 | 97.81 | 2.43 | |
| 20 | 99.95–101.91 | 0.24–1.25 | 100.23 | 0.38 | 96.26–97.18 | 1.12–2.60 | 98.74 | 0.89 | |
| B[a]P | 5 | 98.80–100.52 | 1.03–1.87 | 99.96 | 0.25 | 98.79–101.00 | 1.20–3.76 | 100.60 | 0.68 |
| 10 | 98.31–100.29 | 1.31–3.92 | 100.48 | 0.91 | 96.86–98.77 | 0.75–2.95 | 99.60 | 0.64 | |
| 20 | 99.98–111.30 | 0.96–8.75 | 100.41 | 0.36 | 97.84–99.72 | 0.65–1.27 | 99.86 | 0.48 | |
| I[c,d]P | 5 | 98.92–102.63 | 1.13–4.87 | 99.76 | 0.75 | 91.83–93.76 | 0.64–1.77 | 94.44 | 1.71 |
| 10 | 98.25–101.87 | 1.94–4.27 | 100.27 | 0.38 | 94.07–96.76 | 2.29–2.85 | 98.19 | 1.47 | |
| 20 | 101.47–105.78 | 1.38–3.31 | 101.17 | 1.55 | 94.20–95.55 | 1.97–2.44 | 97.54 | 1.69 | |
| D[a,h]A | 5 | 99.85–105.53 | 0.78–7.00 | 101.37 | 0.71 | 94.62–96.16 | 1.53–7.36 | 98.88 | 1.65 |
| 10 | 100.32–107.50 | 0.51–5.29 | 100.92 | 0.63 | 98.53–104.07 | 1.98–3.86 | 99.82 | 0.89 | |
| 20 | 102.97–111.82 | 2.88–6.44 | 103.99 | 3.43 | 99.54–106.69 | 1.04–7.22 | 100.47 | 1.12 | |
| B[g,h,i]P | 5 | 99.23–105.51 | 1.22–7.10 | 101.03 | 1.41 | 92.71–93.11 | 0.61–1.36 | 94.01 | 0.69 |
| 10 | 104.06–109.97 | 2.77–4.49 | 102.13 | 1.93 | 95.60–96.21 | 1.15–2.50 | 97.70 | 1.10 | |
| 20 | 100.83–108.58 | 1.67–5.75 | 101.38 | 0.87 | 98.29–100.09 | 1.18–1.85 | 99.68 | 1.15 | |
Ranged from mean of 5 determinations performed daily for 3 days
Mean of 3 determinations
Relative standard deviation: 100× standard deviation/mean