Figure 2.

Mapping of the USF responsive element. Left, full-length PHA and its mutated or deleted constructs are represented. The numbers indicate the position of the deleted fragments relative to the start of translation. Right, the different constructs were transiently transfected into keratinocytes and NIH-3T3 cells together with or without a USF-1 expression vector. Luciferase activities were assayed as described in the Materials and Methods and are shown as absolute values. Error bars reflect the SD between at least three separate experiments performed in triplicate.