Table 1.
Monoclonal antibodies used
| Name | Targeta | Epitope use for evaluationb | Array sequencesc | Source |
|---|---|---|---|---|
| Ab1 | aa 211-220 Murine p53-beta galactosidase fusion protein expressed in E. coli., P04637 | RHSVVVP | RHSVV, RHSVVV |
Millipore/Sigma Cat# CBL404 |
| Ab8 | Bacterially expressed full-length human p53, P04637 | SDLWKLL | SDLWKLL, SDLWKL |
ThermoFisher Cat# MA1-19055 |
| 4C1 | External domain of human TSH receptor., P16473 | QAFDSHY | LQAFDS, QAFDSH, FDSHYD |
GeneTex Cat# GTX47974 |
| DM1A | aa 426-450 - human brain α-Tubulin., Q71U36 | LEKDYEE | AALEKDY, ALEKDYE, LEKDYEE |
Millipore/Sigma Cat# 05-829 |
| Lnkb2 | Human IL-2, P60568 | PLEEVLN | PLEEVLN |
Absolute Antibody Cat# Ab00232-1.1 |
| TF3B5 | Aa 1242-1255 C-terminus human ErbB-2 (HER2), P04626 | PEYLGLD | None |
ThermoFisher Cat# Ma5-13675 |
| C3 |
aa 251-450 N-terminal extracellular domain human ErbB-2 (HER2), |
SLPNPEG | None |
Santa Cruz Bio Cat# SC-377344 |
| 9E10.3 | aa 408-439 C-terminus of human c-myc, P01106 | KLISEED | None |
ThermoFisher Cat# MA5-12080 |
aThe targets/antigens the mAbs were raised to. Each target description is followed by a uniport reference number
bThese epitopes were used as the cognate epitope sequence in determining the rank of binding out of 1 million random sequences, see Table 2. Note that because there is no isoleucine on the array, I was substituted for V in mAb 9E10.3 when predicting ranks.
cThe sequences given here were those purposely synthesized on the peptide array in order to evaluate binding to the cognate epitope. TF3B5, C3 and 9E10.3 epitopes were not on the array. Cognate sequences were excluded from the dataset used to train the neural network models.