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. 2024 Jul 8;16(8):1930–1956. doi: 10.1038/s44321-024-00096-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — (A) Statistical analysis of LVSP in mice treated with the solvent DMSO or FR (10 µg/mouse i.p., Monday to Friday) during exposure to Nx (21% O₂, DMSO: n = 8, FR: n = 7) or SuHx (10% O₂, DMSO: n = 10, FR: n = 10) for 3 weeks. (B, C) Statistical analysis of relative Orai1 (B) and TRPC1 (C) mRNA expression in native mPASMCs (n = 8) and mPASMCs treated with solvent DMSO or FR (10⁻⁶M) with or without additional PDGF (40 ng/ml) + 5-HT (10⁻⁶M) stimulation for 12 h, each n = 6 normalized to 18 S housekeeping gene, ns indicate different wells derived from at least two different passages. (D) Amount of TUNEL⁺ CD31⁺ mLECs after 2 days without treatment (n = 6) or with DMSO (n = 6) or FR (10⁻⁶M, n = 6) treatment. (E) Statistical analysis of LVSP in mice treated with the solvent DMSO (n = 9) or FR (10 µg/mouse i.p., Monday to Friday, n = 9) in the last 2 weeks of 5 weeks SuHx exposure. (F, G) vWF/α-SMAC staining of PAs in lung sections from SuHx-DMSO (F) and SuHx-FR-treated mice (G), scale bars: 20 µm. (H) Statistical analysis of collagen deposition in the right ventricle in mice treated with the solvent DMSO (n = 9) or FR (10 µg/mouse i.p., Monday to Friday, n = 9) in the last 2 weeks of 5 weeks SuHx exposure. Data information: Values are expressed as mean ± SEM. (A–D) One-way ANOVA, Tukey’s post hoc test, (E, H) Unpaired student’s t-test. Source data are available online for this figure.