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. 2024 Jul 8;16(8):1930–1956. doi: 10.1038/s44321-024-00096-0

Figure 2. FR abolishes 5-HT-induced pulmonary vasoconstriction, which is mediated via Gα11 and Gαq.

Figure 2

(A) Dose–response curve of 5-HT (10−9 M–10−5 M) after pre-incubation with the solvent DMSO (n = 6), the 5-HT2A/2C receptor antagonist Ket (10−6 M, n = 4) or FR (10−6 M, n = 4) in isometric force measurements of mouse PAs. (B) Statistical analysis of vasoconstriction at the highest 5-HT concentration applied (10−5 M) in the dose–response curve in (A). (C) DMR dose–response curve of α-methyl-5-HT (10−14 M–10−6 M) in native mPASMC (n = 3) and mPASMC transduced with control virus (sh-ctrl, n = 3) or virus containing a sh-G11 (n = 3), sh-Gq (n = 3) or sh-G11 + sh-Gq (n = 3) RNA plasmid. As positive control FR (10−6 M, n = 4) was applied to native cells before the highest α-methyl-5-HT (10−6 M) concentration, DMR responses were analyzed in three independent experiments and normalized to Gq-independent signals induced by EGF. Data information: Values are expressed as mean ± SEM. (B) One-way ANOVA, Tukey’s post hoc test. Source data are available online for this figure.