Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 8;16(8):1930–1956. doi: 10.1038/s44321-024-00096-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A, B) Phase contrast microscopy pictures of intrapulmonary mouse vessels after solvent DMSO (A) or FR (10⁻⁶M) (B) application. Numbers indicate time points shown in the graph (1–3), scale bar: 20 μm. (C) Statistical analysis of small intrapulmonary vessel relaxation evoked by DMSO (n = 8 intrapulmonary PAs) or FR (10⁻⁶M, n = 7 intrapulmonary PAs). (D, E) Phase contrast microscopy pictures of intrapulmonary vessels after solvent DMSO (D) or FR (10⁻⁶M, E) application in precision-cut lung slices of human subjects, arrows indicate PAs, scale bar: 200 µm. (F) Statistical analysis of vascular area changes evoked by DMSO (n = 5 intrapulmonary PAs) or FR (10⁻⁶M, n = 8 intrapulmonary PAs). (G) Original traces of PAP in the IPL mouse model during perfusion with 5-HT (10⁻⁶M) and DMSO or FR (10⁻⁶M). (H) Statistical analysis of DMSO (n = 6 mice) or FR (10⁻⁶M, n = 6 mice)-induced PAP decrease. (I) Statistical analysis of 5-HT (5 × 10⁻⁷M)-induced constriction amplitude in healthy mice housed under Nx (21% O₂, n = 22) conditions or mice with Hx-induced PH (n = 12). (J) Statistical analysis of DMSO (n = 6) or FR (10⁻⁶ M, n = 6)-dependent pulmonary vasorelaxation after constriction with 5-HT (5 × 10⁻⁷ M) in mice with pre-existing Hx-induced PH. (K) Schematic diagram of the procedure for acute FR application and hemodynamic analysis in mouse in vivo. (L) Statistical analysis of basal RVSP 1 h after DMSO (n = 9) or FR (2.5 µg/mouse, n = 8) i.t. application in mice with pre-existing Hx-induced PH. (M) Statistical analysis of right ventricular pressure response to acute 5-HT i.v. bolus injection (5 × 10⁻³ M, 10 µl) in these mice (DMSO: n = 9; FR: n = 8). Data information: Values are expressed as mean ± SEM. (C, F, H, I, J, L, M) Unpaired student’s t-test. Source data are available online for this figure.