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. 2024 Jul 8;16(8):1930–1956. doi: 10.1038/s44321-024-00096-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV1 — (A) Original traces of 5-HT dose–response curves (10⁻⁹M–10⁻⁵M) after pre-incubation with DMSO, Ket (10⁻⁶M) or FR (10⁻⁶ M) in PAs. (B) PCR analysis of Gq protein subtypes in native mPASMCs and mPASMCs transduced with lentiviral sh-G11, sh-Gq RNA (negative controls). Murine lung tissue was used as positive control. (C–F) Statistical analysis of relative Gα₁₁ (C), Gα_q (D), Gα_i (E), and Gα_s (F) mRNA expression in native mPASMCs (n = 3) and mPASMCs transduced with lentivirus (sh-control (ctrl), sh-G11, sh-Gq RNA or both, n = 3 independent experiments normalized to 18S housekeeping gene. (G, H) Original Western Blot (G) and analysis (H) of Gα_q/11/14 protein expression of native mPASMCs and mPASMCs transduced with lentivirus (sh-control (ctrl), sh-G11, sh-Gq RNA or both, n = 3 independent experiments). GAPDH was used as housekeeper. Data information: Values are expressed as mean ± SEM. (C–F, H) One-way ANOVA, Tukey’s post hoc test. Source data are available online for this figure.