Figure 11.

A₃R-directed immunohistochemical labeling of the olfactory bulb. (A) Low-power microscopic image of control OB showing (B) strong A₃R labeling in periglomerular neurons and (C,D) apical dendrites of mitral and tufted cells. (E) A₃R-ir labeling in the EAE section shows (F) numerous A₃R-ir infiltrates in the ONL and conspicuous labeling of (G) periglomerular neurons and (H) granular cells. (I) Double immunofluorescence directed against A₃R (red) and Iba1 (green) shows dominant expression in infiltrated macrophages and ameboid microglia (arrows), and (J) the absence of A₃R expression in ramified microglia in the EPL. (K,L) Double immunofluorescence directed to A₃R (red) and GFAP (green) shows the absence of A3R expression in astrocytes. (M) Very pronounced A₃R labeling of soma and apical dendrites of granule cells (arrow). (N) PCC value for A₃R and Iba1 signal overlap in control (black bars) and EAE (red bars) sections. GCL, granule cell layer; GL, glomerular layer; EPL, external plexiform layer; IPL, internal plexiform layer; MCL, mitral cell layer; ONL, olfactory nerve layer; RMS—rostral migratory stream. Scale bar: 500 μm in (A,E); 50 μm in (B,C,D,F,G,H): 20 μm in (I–M). Significance within the graph: ***p < 0.001.