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. 2024 Jul 30;18:1407975. doi: 10.3389/fncel.2024.1407975

Figure 8.

Figure 8

eN/CD73-directed immunohistochemical labeling of the olfactory bulb. (A–C) The pattern of eN/CD73-ir labeling in the ONL, GL, and EPL implies dominant astrocyte localization. (D–I) eN/CD73-ir in EAE sections. (E) Intense labeling of OEC cells and (F) astrocytes in the GL, EPL, and GCL astrocytes. (H) Sporadic labeling of tufted cells (arrows). (J) Double immunofluorescence directed to eN/CD73 (blue) and GFAP (green) showed astrocytic localization. (K–L) Double immunofluorescence directed to eN/CD73 (blue) and Iba1 (red) demonstrates induction of eN/CD73 in reactive microglia. (M) Double immunofluorescence directed to eN/CD73 (blue) and NeuN (green), indicating a possible presynaptic localization in mitral and tufted cell dendrites. (N) Pearson’s correlation coefficient (PCC) shows a degree of overlap between the fluorescence signals. GCL, granule cell layer; GL, glomerular layer; EPL, external plexiform layer; IPL, internal plexiform layer; MCL, mitral cell layer; ONL, olfactory nerve layer, RMS—rostral migratory stream. Scale bar: 500 μm in (A,D); 100 μm in (C,F); 50 μm in (B,E,G,H,I); 20 μm in (J–L). Significance is shown within the graph: **p < 0.01.