Figure 9.

A₁R-directed immunohistochemical labeling of the olfactory bulb. (A–C) Control sections were immunostained for A₁R. (B) The somatic A₁R-ir was seen in the cell bodies of periglomerular neurons and (C) tufted cells and mitral cells. The frame enlarged in C′ shows A₁R-ir mitral cells. (D) A₁R-ir in the EAE section shows increased ir in the ONL, (E) EPL and MCL, and (F) GCL. (F′) High-power magnification of the area enclosed by rectangle in (F). (G–H) Double immunofluorescence directed to A₁R (red) and NeuN (green) shows (G,H) the somatic localization of A₁R in perglomerular neurons and (I–J) in granule cells. Asterisks in (I) denote A₁R-ir mitral cells which do not express NeuN. (K–L) Double immunofluorescence directed to A₁R (red) and Vglut1 (blue), which show prominent overlap of the signals in (K) EPL and (L) IPL. (M) Pearson correlation coefficient (PCC) reflecting an increase in A₁R/VGlut1 overlap in EAE in comparison to control. GCL, granule cell layer; GL, glomerular layer; EPL, external plexiform layer; IPL, internal plexiform layer; MCL, mitral cell layer; ONL, olfactory nerve layer, RMS—rostral migratory stream. Scale bar: 500 μm in (A); 200 μm in (D); 100 μm in (B,C,E,F); 50 μm in (C′,F′,G,I,K,L); 20 μm in (H,J). Significance within the graph: *p < 0.01.