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. 2024 May 9;23(8):e14190. doi: 10.1111/acel.14190

FIGURE 5.

FIGURE 5

Age impacts microbiota‐dependent immunogenic signatures after treatment with antibiotics. (a) A schematic representation of antibiotic experiment in immunogenicity. Mice were exposed to broad‐spectrum antibiotics (Abx) in drinking water for 7 days and then were switched to regular drinking water for an additional 7 days. (b) TLR4 and TLR5 activity in feces after Abx treatment (D0); (c) TLR 4 and (d) TLR5 activity in feces, proximal colon (PC) contents, and mucosal scrapings after recovery period (D7), assessed using human embryonic kidney (HEK)‐blue‐mTLR4 and HEK‐blue‐mTLR5 cells. Data were normalized within each independent experiment and relativized to young control at AU =1. (e) Top 25 genera correlated with TLR4 and TLR5 activity (Spearman rank correlation). Data correspond to n = 3 cohorts with n = 9–15 per group.