. 2024 May 29;23(8):e14194. doi: 10.1111/acel.14194

TABLE 2.

Maternal Age and Offspring Blood DNA methylation.

CpG ^a	Newborn blood DNA methylation							Childhood blood DNA methylation
CpG ^a	Estimate	SE	p‐Value	Chr	Position	Gene	Direction ^b	Estimate	SE	p‐Value	Direction ^c
cg00528572	0.001341	0.000191	2.41E‐12	11	92,703,433	MTNR1B	++++++−+++−++++++	0.00097	0.00033	0.003	−+++
cg07105285	0.001011	0.000157	1.13E‐10	11	92,702,663	MTNR1B	+++++++−++−++++++	0.00034	0.0002	0.097	−−++
cg03970229	0.000626	0.000106	3.42E‐09	11	92,702,507	MTNR1B	+++++++++++++++++	0.00033	0.00015	0.027	−+++
cg03292743	−0.00095	0.000162	5.03E‐09	2	74,642,838	C2orf81	−−−−−−−−−−−−+−+−−	−0.00042	0.00027	0.129	−
cg15559898	0.000622	0.000107	6.53E‐09	11	92,703,185	MTNR1B	+++++++−++−++++++	0.00048	0.00018	0.008	−+++
cg07609862	0.001325	0.00023	8.77E‐09	11	92,702,912	MTNR1B	+++++++−++−++++++	0.00054	0.00024	0.023	−+++
cg26352652	0.000857	0.000153	2.17E‐08	12	133,013,600		++?++++−−−+++++++	0.00068	0.00025	0.007	++++
cg01722932	0.000608	0.000111	4.62E‐08	11	92,702,653	MTNR1B	+++++++−++−++++++	0.00026	0.00014	0.064	−+++
cg12082025	0.000674	0.000128	1.44E‐07	19	1,064,218	ABCA7	+++++−−−++−++++−+	−0.00001	0.00007	0.843	+−++
cg14898611	0.000684	0.00013	1.53E‐07	7	25,608,673		−++−+++++++++++++	0.00064	0.00022	0.003	−+++
cg00538458	0.000928	0.000178	1.83E‐07	16	22,959,868		−+++−++++++++++++	0.00072	0.0003	0.015	−+−+
cg14503935	0.000712	0.00014	3.53E‐07	12	131,864,894		−++−++++−?+++++++	0.00022	0.00024	0.372	+++−
cg12965344	0.000375	0.000074	3.91E‐07	19	48,898,160	GRIN2D	−++++++++−+++++++	0.00043	0.00014	0.002	+++−
cg22968966	0.00086	0.000171	5.04E‐07	16	22,959,875		++−+−++++++++++++	0.0008	0.00028	0.004	−+++
cg07887168	0.000561	0.000112	5.28E‐07	16	4,263,893	SRL	++++−+++++++−−++?	−0.00009	0.00015	0.563	+−−+
cg26709300	−0.00041	0.000082	5.80E‐07	16	30,106,682	YPEL3, BOLA2B	−−+−−−−+−−−+−+−−−	−0.00037	0.00014	0.008	−
cg08918020	0.000386	0.000078	8.25E‐07	20	30,225,706	COX4I2	−++++++−+++++++++	0.00041	0.00016	0.011	++++
cg16702083	0.001556	0.000316	8.46E‐07	20	62,328,427	RTEL1‐TNFRSF6B	++++−++−++++−++++	0.00229	0.00053	0.00001	++++
cg05803237	0.000508	0.000103	8.97E‐07	11	92,702,628	MTNR1B	++++++++++−++++++	0.00006	0.00014	0.642	−−++
cg24536250	0.000611	0.000125	9.43E‐07	5	50,218,625		+−−+−+++++++++++−	0.00021	0.00021	0.322	−+−+
cg24354818	0.001391	0.000287	1.27E‐06	20	62,328,094	RTEL1‐TNFRSF6B	++++−++−++++−++++	0.00177	0.00037	0.000002	++++
cg01817364	−0.00041	0.000084	1.45E‐06	5	43,037,411		−−−−−−++−+−−−−−−−	0.00005	0.0002	0.8	−−+−
cg24129222	−0.00097	0.000202	1.68E‐06	2	74,643,251	C2orf81	−−−−−−−−−−−−+−−−−	−0.00039	0.00036	0.279	−
cg12600858	0.000643	0.000134	1.76E‐06	11	92,702,530	MTNR1B	+++++++++++++++−+	0.00049	0.00023	0.03	−+++
cg13728287	0.000626	0.000131	1.90E‐06	7	25,608,634		++−−++++++−++++++	0.00059	0.00021	0.005	−+++
cg09405380	0.000453	0.000095	1.93E‐06	19	19,730,109	PBX4	−+++++−−+++++++++	−0.00003	0.00013	0.817	−+−−
cg06741367	0.000857	0.00018	1.94E‐06	2	128,453,108		+++++++++++++++++	0.00053	0.00023	0.022	++++
cg24488001	0.00053	0.000112	2.42E‐06	1	238,644,629	LINCO1139	++−+−+−−+++++?+??	−0.00004	0.00016	0.79	+−−+
cg18473455	0.000755	0.00016	2.52E‐06	19	47,333,922	SNAR‐E	++−+++++++++−++++	−0.00013	0.00032	0.681	−+−−
cg23773946	0.001299	0.000277	2.76E‐06	20	62,327,968	RTEL1‐TNFRSF6B	++++−++−+−++++++?	0.00242	0.00062	0.00009	++++
cg05372495	0.000413	0.000088	2.88E‐06	19	1,063,624	ABCA7	++−+++−++++++++++	0.00002	0.00007	0.719	+−++
cg15829826	−0.00034	0.000072	3.13E‐06	11	65,153,860	FRMD8	−−−−−−+−−−−−+−−−−	−0.00011	0.00007	0.125	−−−+
cg25594486	0.000589	0.000127	3.51E‐06	19	51,165,441	SHANK1	++++−++++++++++++	0.00073	0.00029	0.013	−++−

Note: The 33 CpGs associated at birth (P_FDR < 0.05) with original p‐values shown above. Maternal age was modeled in years and adjusted for cell types, maternal smoking, maternal education, maternal race/ethnicity/ancestry, parity, batch, and selection (as applicable, see Data S1). These 33 CpGs were also reviewed in childhood analyses adjusting for the same covariates. Estimates represent the difference in DNA methylation levels (i.e., beta values) per year of maternal age. See Table S3 for full annotation including location information and heterogeneity statistics.

Abbreviations: Chr, chromosome; SE, standard error.

^{^a}

Bolding indicates remained significant (P_FDR < 0.05) in model 3 (after additional adjustment for maternal BMI).

^{^b}

Cohorts listed in the following order for newborn associations: EAGeR, Gen3G, DCHS, ALSPAC, NORTHPOP, GenR, POSEIDON, EARLI, MARBLES, CHAMACOS, INMA, MoBa1, MoBa2, MoBa4, MoBa8, Upstate KIDS, and CHS.

^{^c}

Cohorts listed in the following order for childhood associations: Upstate KIDS, HELIX, MMNP, and PMMST.