FIGURE 5.

Senescent cell GH suppresses CXCL1 by inhibiting NFκB activity, and changes cytokine SASP content. (a, b) hNCC were infected with either lenti‐shGH or lenti‐shScr, treated with 50 μM etoposide (Etop) to induce senescence or with DMSO as control (C) then analyzed 6 days later. Western blot of CXCL1 in (a) cells and (b) culture medium (in duplicate). EM, empty medium. (c, d) Western blot of organoids 1 week after infection with (c) lenti‐shGH or lenti‐shScr and (d) lentiGH or lentiV. (e) Western blot of hNCC treated with 50 μM etoposide for 2 days, sorted for senescence on Day 3, and treated with GH for an additional 24 h. Representative blots from at least three independent experiments are shown. ImageJ quantification of western blots is depicted in Figure S8. Schematic representations depict experimental design.