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. 2024 Jul 9;10(15):e34321. doi: 10.1016/j.heliyon.2024.e34321

Fig. 2.

Fig. 2

hADSCs-Exo and antioxidants protected HaCaT cells from UVB. a Flow cytometric analysis of apoptotic cells in control group and UV group. b-c Images of senescence-associated SA-β-gal staining of proliferating and senescent HaCaT cells. Control group and UV group (b). hADSCs-Exo and antioxidants protected HaCaT cells from UVB-induced apoptosis in SA-β-gal staining (c). d The migratory properties of HaCaT cells were analyzed using the Transwell migration assay with Transwell filter chambers. e WB Western blot. In UV group, the level of COL17 downregulated and MMP3 levels upregulated by UVB. hADSC-Exo and five antioxidants transfection rescued the expression level of COL17 and MMP3 to different extents. Full-length blots/gels are presented in Supplementary original image of the blotting. Quantification of COL17 and MMP3, normalized to GAPDH levels, and values were plotted. Idebenone (IDE), chlorogenic acid (CGA), vitamin C (VC), VE, zinc (Zn). n = 3. Control, non-exposed HaCaT cells. UV, UVB-exposed HaCaT cells. IDE + EXO, hADSC-Exo and Idebenone-pretreated UVB-exposed HaCaT cells. CGA + EXO, hADSC-Exo and chlorogenic acid-pretreated UVB-exposed HaCaT cells. VC + EXO, hADSC-Exo and vitamin C-pretreated UVB-exposed HaCaT cells. VE + EXO, hADSC-Exo and vitamin E-pretreated UVB-exposed HaCaT cells. Zn + EXO, hADSC-Exo and zinc-pretreated UVB-exposed HaCaT cells. Differences in eight groups were assessed by Tukey's multiple comparison test one-way ANOVA, error bars represent S.E.M. Compared with control group, markered with *p < 0.05, **p < 0.01, ***p < 0.001. Compared with UV group, markered with #p < 0.05,##p < 0.01,###p < 0.001,####p < 0.0001.