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. 2024 Jul 9;10(15):e34321. doi: 10.1016/j.heliyon.2024.e34321

Fig. 4.

Fig. 4

hADSC-Exo and VE delivery to synergistically against the UVB-induced photoaging in 3D skin model. a-c HE staining of 3D skin model. Representative images in 3D skin model constructs. Stratum Basale (SB), Stratum Corneum (SC), Stratum Granulosum (SG), Stratum Spinosum (SS). (a). Analysis of the thickness of the epidermal layer of 3D skin model in HE staining sections (b). Thickness quantifications of the stratum epidermis (c). d-e Masson staining. Representative images in collagen deposition (blue, d). Quantification of 3D skin model for fibrosis with representative Masson trichrome image (e). f and i Representative photographs of TNF-α, immunostaining. Scale bar = 50 μm (f). Quantitative analysis of TNF-α (i). g and j Representative photographs of COL-17, immunostaining. Scale bar = 50 μm (g). Quantitative analysis of COL-17 (j). h and k Representative photographs of MMP3, immunostaining. Scale bar = 50 μm (h). Quantitative analysis of MMP3 (k). Control, non-exposed 3D skin model. UV, UVB-exposed 3D skin model. UV + VE, VE-pretreated UVB-exposed 3D skin model. UV + EXO, hADSC-Exo-pretreated UVB-exposed 3D skin model. UV + VE + EXO, hADSC-Exo and VE-pretreated UVB-exposed 3D skin model. Differences in five groups were assessed by Tukey's multiple comparison test one-way ANOVA, error bars represent S.E.M. Compared with control group, markered with *p < 0.05, **p < 0.01, ***p < 0.001. Compared with UV group, markered with #p < 0.05,##p < 0.01,###p < 0.001,####p < 0.0001.