Fig. 5.

hADSC-Exo and VE delivery to synergistically against the UVB-induced photoaging in photoaged mouse model. a Schematic flowchart of the experiment of UVB-exposed and shaved in photoaged mouse model. b The gross appearances of skin damaged by photoaging in each group. Mice were randomly grouped. Six animals were in each group. c and e HE staining of photoaged mouse model. Representative images in photoaged mouse model constructs (c). Thickness quantifications of the stratum epidermis of photoaged mouse model in HE staining sections (e). d and f Masson staining. Representative images in collagen deposition (blue, d). Quantification of photoaged mouse model for fibrosis with representative Masson trichrome image (f). g The changes of skin moisture content of the photoaged mouse model. h and k Representative photographs of TNF-α, immunostaining. Scale bar = 50 μm (h). Quantitative analysis of TNF-α (k). i and l Representative photographs of COL-17, immunostaining. Scale bar = 50 μm (i). Quantitative analysis of COL-17 (l). j and m Representative photographs of MMP3, immunostaining. Scale bar = 50 μm (j). Quantitative analysis of MMP3 (m). Control, non-exposed photoaged mouse skin. UV, UVB-exposed photoaged mouse skin. UV + VE, VE-pretreated UVB-exposed photoaged mouse skin. UV + EXO, hADSC-Exo-pretreated UVB-exposed photoaged mouse skin. UV + VE + EXO, hADSC-Exo and VE-pretreated UVB-exposed photoaged mouse skin. Differences in five groups were assessed by Tukey's multiple comparison test one-way ANOVA, error bars represent S.E.M. Compared with control group, markered with *p < 0.05, **p < 0.01, ***p < 0.001. Compared with UV group, markered with #p < 0.05,##p < 0.01,###p < 0.001,####p < 0.0001. Three mice were randomly selected from each group for histological examination.