FIG. 8.

In vivo depletion of CD4⁺ T cells and protective immunity against HSV challenge. Each group of inbred BALB/c mice (n = 5) was immunized with 20 μg of IL-8 cDNAs (A) or RANTES cDNAs (B) and/or gD DNA vaccine (30 μg). After 3 weeks following the initial DNA immunization, one group of mice was administered 100 μl of anti-CD4 (clone GK1.5) ascites fluid i.p. on days −3, 0, and 3 of viral challenge. Mice were subsequently challenged i.vag. with 4 LD₅₀ of HSV-2 (strain 186) and then checked for 15 days to determine survival rates. Asterisks indicate results that are statistically significant at a P value of <0.05 using ANOVA compared to that for naive control mice. The experiments were repeated with similar results.