Fig. 2 |. JADE1 recruits HBO1 to the nucleosome.

a, Schematic of HBO1 complex subunits with dot lines indicating contacts between subunits. The catalytic MYST domain and histone-binding zinc finger domains are labeled. The naturally occurring splice variant JADE1S is indicated by an arrow. b, JADE1 and JADE1S proteins were stably expressed as 3Flag-2Strep tagged versions from the AAVS1 safe harbor locus in K562 cells and purified from soluble nuclear extracts by tandem affinity chromatography. Silver-stained SDS–PAGE of biotin-eluted fractions is shown with associated protein bands labeled. c, Western blot of the purified JADE1- and JADE1S-containing HBO1 complexes with proteins detected by indicated antibodies. d, HAT assays using the purified HBO1 complexes from b and c with recombinant NCPs unmodified, carrying one (asymmetric) or two (symmetric) H3K4me3 tails (red dots). e, Cartoon summarizing results in d. Kac, acetylated lysine. f, Overlay of ¹H,¹⁵N HSQC spectra of JADE1_PZP in the presence of increasing amounts of H3_1–12 peptide. Spectra are colored according to the protein:peptide molar ratio. g, Binding curves used to determine $K_{\mathrm{d}}$ by tryptophan fluorescence. $K_{\mathrm{d}}$ is represented as average ± s.d. from three independent experiments (n = 3). h, Overlay of ¹H,¹⁵N HSQC spectra of JADE1_PZP in the presence of increasing amounts of H3K4me3_1–12 peptide. Spectra are colored according to the protein:peptide molar ratio. i, Binding curves used to determine $K_{\mathrm{d}}$ by NMR. $K_{\mathrm{d}}$ values are represented as average ± s.d. from four signals. j,k, Models of the chromatin association mechanism and function of the JADE1–HBO1 complexes.