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Effects of neuraminidase on cells. CEM×174 SIV-SEAP cells were treated for 6 h with 40 mU of neuraminidase, which is 10 times the largest amount of neuraminidase present during the measurement of neuraminidase-treated virus in the infectivity assay. These cells were then washed with neuraminidase-free medium and infected with either mock- or neuraminidase-treated SIVmac239. SEAP activity in the medium was assayed at approximately 60 h postinfection as described in Materials and Methods.
