(A) Dot plot of Atg5 gene expression across the pseudotime (Figure 2D).
(B) Experimental paradigm to knock out Atg5 in DG NSCs in vivo. Hopx-CreERT2::mTmG Atg5wt/wt and Hopx-CreERT2::mTmG Atg5Flx/Flx mice were administered tamoxifen at P1 and analyzed at P7 and P14.
(C) Sample confocal images of GFP+HOPX+ cells in the DG at P7 in Hopx-CreERT2::mTmG Atg5wt/wt and Hopx-CreERT2::mTmG Atg5Flx/Flx mice (left panels). Boxed area is shown at higher magnification (right panels) to view GFP+HOPX+MCM2+ cells (arrows) and GFP+HOPX+MCM2− cells (arrowheads). Scale bars, 50 μm (left panels) and 25 μm (right panels).
(D) Quantification of the percentage of MCM2+ cells among all GFP+HOPX+ cells in the DG at P7. Each dot represents data from one mouse. Values represent mean ± SEM (n = 4 mice; **p < 0.01; unpaired t test).
(E) Quantification of the number of GFP+HOPX+ cells in the DG at P7. Each dot represents data from one mouse. Values represent mean ± SEM (n = 4 mice).
(F) Sample confocal images of GFP+HOPX+ cells in the DG at P14 in Hopx-CreERT2::mTmG Atg5wt/wt and Hopx-CreERT2::mTmG Atg5Flx/Flx mice (left panels). Boxed area is shown at higher magnification (right panels) to view GFP+HOPX+MCM2+ cells (arrows) and GFP+HOPX+MCM2− cells (arrowheads). Scale bars, 50 μm (left panels) and 25 μm (right panels).
(G) Quantification of the percentage of MCM2+ cells among all GFP+HOPX+ cells in the DG at P14. Each dot represents data from one mouse. Values represent mean ± SEM (n = 4 mice; *p < 0.05; unpaired t test).
(H) Quantification of the number of GFP+HOPX+ cells in the DG at P14. Each dot represents data from one mouse. Values represent mean ± SEM (n = 4 mice; *p < 0.05; unpaired t test).
Also see Figure S6.