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. Author manuscript; available in PMC: 2024 Aug 13.
Published in final edited form as: Cell Rep. 2024 Jun 8;43(6):114339. doi: 10.1016/j.celrep.2024.114339

Figure 6. Blocking autophagy attenuates the NSC transition to quiescence.

Figure 6.

(A) Dot plot of Atg5 gene expression across the pseudotime (Figure 2D).

(B) Experimental paradigm to knock out Atg5 in DG NSCs in vivo. Hopx-CreERT2::mTmG Atg5wt/wt and Hopx-CreERT2::mTmG Atg5Flx/Flx mice were administered tamoxifen at P1 and analyzed at P7 and P14.

(C) Sample confocal images of GFP+HOPX+ cells in the DG at P7 in Hopx-CreERT2::mTmG Atg5wt/wt and Hopx-CreERT2::mTmG Atg5Flx/Flx mice (left panels). Boxed area is shown at higher magnification (right panels) to view GFP+HOPX+MCM2+ cells (arrows) and GFP+HOPX+MCM2 cells (arrowheads). Scale bars, 50 μm (left panels) and 25 μm (right panels).

(D) Quantification of the percentage of MCM2+ cells among all GFP+HOPX+ cells in the DG at P7. Each dot represents data from one mouse. Values represent mean ± SEM (n = 4 mice; **p < 0.01; unpaired t test).

(E) Quantification of the number of GFP+HOPX+ cells in the DG at P7. Each dot represents data from one mouse. Values represent mean ± SEM (n = 4 mice).

(F) Sample confocal images of GFP+HOPX+ cells in the DG at P14 in Hopx-CreERT2::mTmG Atg5wt/wt and Hopx-CreERT2::mTmG Atg5Flx/Flx mice (left panels). Boxed area is shown at higher magnification (right panels) to view GFP+HOPX+MCM2+ cells (arrows) and GFP+HOPX+MCM2 cells (arrowheads). Scale bars, 50 μm (left panels) and 25 μm (right panels).

(G) Quantification of the percentage of MCM2+ cells among all GFP+HOPX+ cells in the DG at P14. Each dot represents data from one mouse. Values represent mean ± SEM (n = 4 mice; *p < 0.05; unpaired t test).

(H) Quantification of the number of GFP+HOPX+ cells in the DG at P14. Each dot represents data from one mouse. Values represent mean ± SEM (n = 4 mice; *p < 0.05; unpaired t test).

Also see Figure S6.