Reagent or resource	Source	Identifier
Antibodies
Mouse monoclonal 9.4A anti-Trio	University of Iowa Developmental Studies Hybridoma Bank	RRID:AB_528494
Mouse monoclonal ADL101 anti-Lamin	University of Iowa Developmental Studies Hybridoma Bank	RRID:AB_528332
Mouse monoclonal 1D4 anti-Fasciclin II	University of Iowa Developmental Studies Hybridoma Bank	RRID: AB_528235
Rabbit Polyclonal Anti-Green Fluorescent Protein (GFP)	Thermo Fisher Scientific	Catalogue no. A-11122
Alexa Fluor 488 AffiniPure Polyclonal Goat Anti-rabbit IgG	Jackson ImmunoResearch Laboratories	RRID: AB_2338046
Cy3 AffiniPure Polyclonal Goat Anti-Mouse IgG	Jackson ImmunoResearch Laboratories	RRID: AB_2338690
Chemicals, peptides, and recombinant proteins
TRIzol reagent	Invitrogen	Catalogue no. 15596018
1-Bromo-3-chloropropane	Scientific Laboratory Supplies	Catalogue no. B9673
2-propanol/isopropanol	Thermo Fisher Scientific	Catalogue no. A416-1
Ethanol 200 proof	Thermo Fisher Scientific	Catalogue no. 04-355-233
Diethyl pyrocarbonate (DEPC)	Sigma-Aldrich	Catalogue no. D5758
Agarose LE, Quick Dissolve	Genesee Scientific	Catalogue no. 20-102QD
Red Safe	iNtRON Biotechnology	Catalogue no. 21141
NaCl	Sigma-Aldrich	Catalogue no. S7653
KCl	Sigma-Aldrich	Catalogue no. P3911
ZnCl2	Sigma-Aldrich	Catalogue no. 208086
Polysorbate 20 (Tween 20)	Fisher Bioreagents	Catalogue no. BP-337-500
Triton X-100	Sigma-Aldrich	Catalogue no. T8787
Tris Base Ultrapure	USBiological Life Sciences	Catalogue no. T8600
Sodium Dodecyl Sulfate	Fisher Bioreagents	Catalogue no.YBP166500
Sodium deoxycholate	Thermo Fisher Scientific	Catalogue no. 89905
NP-40	Thermo Fisher Scientific	Catalogue no. 85124
Dithiothreitol (DTT)	Thermo Fisher Scientific	Catalogue no. R0861
Bromophenol Blue	Thermo Fisher Scientific Chemicals	Catalogue no. A18469-18
Glycerol	USBiological Life Sciences	Catalogue no. G8145
RNaseOUT	Invitrogen	Catalogue no. 10777019
p-Coumaric acid	Sigma-Aldrich	Catalogue no. C9008
Luminol	Sigma-Aldrich	Catalogue no.A8511
Acetic Acid, Glacial	Thermo Fisher Scientific	Catalogue no. A38-212
EDTA	USBiological Life Sciences	Catalogue no. E2210
Paraformaldehyde	Electron Microscopy Sciences	Catalogue no. 15713
VECTASHEILD mounting medium	Vector Laboratories Inc.	REF#H-1000
Normal Goat Serum	Jackson ImmunoResearch Laboratories	RRID:AB_2336990
Diethyl Ether Anhydrous (stabilized with BHT)	Tokyo Chemical Industry (TCI) America	Catalogue no. D3497
Halocarbon oil 27	Sigma-Aldrich	Catalogue no. H8773
Critical commercial assays
DNase I, Amplification Grade	Invitrogen	Catalogue no. 18068015
M-MLV Reverse Transcriptase	Invitrogen	Catalogue no. 28025013
Taq DNA Polymerase (1000U)	Qiagen	Catalogue no. 201205
QuantiTect SYBR Green PCR Kit	Qiagen	Catalogue no. 204145
MicroAmp Fast Optical 96-Well Reaction Plate with Barcode, 0.1 ml	Applied Biosystems	Catalogue no. 4346906
MicroAmp Optical Adhesive Film	Applied Biosystems	Catalogue no. 4311971
4–20% Mini-PROTEAN TGX Stain-Free™ Protein Gels	Bio-Rad	Catalogue no. 4568093
Nitrocellulose Membrane, 0.2 µm	Bio-Rad	Catalogue no. 1620112
iScript Reverse Transcriptase Supermix	Bio-Rad	Catalogue no. 1708841
Phusion High Fidelity PCR	Thermo Fisher Scientific	Catalogue no. F530S
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	Catalogue no. 23225
SuperFrost Plus slides	Thermo Fisher Scientific	Catalogue no. 12-550-15
Il90p-Experimental models: Organisms/Strains
D. melanogaster: w-;;Nab2pex41 (Control);	(Pak et al., 2011)	N/A
D. melanogaster: w-;;Nab2ex3 (Nab2null); (Zygotic loss of Nab2)	(Pak et al., 2011)	N/A
D. melanogaster: w-;;Mettl3null;	(Lence et al., 2016)	N/A
D. melanogaster:YT521-B∆N	(Lence et al., 2016)	N/A
D. melanogaster: Ythdf∆YTH (Ythdf0)	(Worpenberg et al., 2021)	N/A
D. melanogaster:w1118;;Df(3R)BSC655 (ythdf deficiency)	Bloomington Drosophila Stock Company	BDSC:26507
D. melanogaster: y1w67c23;UAS-mcd8::GFP, 201Y-Gal4;;	Bloomington Drosophila Stock Company	BDSC:64296
D. melanogaster:w*;Ckac305a-Gal4	Bloomington Drosophila Stock Company	BDSC:30829
D. melanogaster: y1w*;UAS-mcd8::GFP;;	Bloomington Drosophila Stock Company	BDSC:5137
D. melanogaster: w*;;;OK107-Gal4	Bloomington Drosophila Stock Company	BDSC:854
D. melanogaster:y1w*:UAS-trio-GEF1-myc(Trio-GEF1 domain; AAs 1287-1583 tagged at the 3′end with eight copies of the tag MYC)	Bloomington Drosophila Stock Company	BDSC:9133
D. melanogaster: y1w*:UAS-trio-GEF2-myc(Trio-GEF2 domain; AAs 1945-2246 tagged at the 3′end with eight copies of the tag MYC)	Bloomington Drosophila Stock Company	BDSC:9134
D. melanogaster:w*;;ppk-Gal4	Bloomington Drosophila Stock Company	BSDC:32079
D. melanogaster:w*;UAS-Rac1	Bloomington Drosophila Stock Company	BSDC:6293
D. melanogaster:w*;UAS-Rho1	Bloomington Drosophila Stock Company	BSDC:58819
Oligonucleotides
RT-PCR trio L Forward: AACAAAACAGAGAGCGCCC	This study	N/A
RT-PCR trio L Reverse: GATGGGCACTGCAGCATAA	This study	N/A
qRT-PCR trio L Intron Retention Forward: 5′-TTAGCCCGCGTCAAGTC-3′	This study	N/A
qRT-PCR trio L Intron Retention Reverse: 5′-CTGCTTGTGCCACCAAAT-3′	This study	N/A
qRT-PCR trio L Properly Spliced Forward: 5′-GTTGTGTTGACAAAAGAGTG-3′	This study	N/A
qRT-PCR trio L Properly Spliced Reverse: 5′-GATGGGCACTGCAGCATAA-3′	This study	N/A
RT-PCR trio M Intron Retention (1) Forward: 5′-CAGCAGTCTCTTCTTCACTAA-3′	This study	N/A
RT-PCR trio M Intron Retention (1) Reverse: 5′-ACTCGGATTGTTGTTTCACTTT-3′	This study	N/A
RT-PCR trio M Intron Retention (2) Forward: 5′-GACTGCGCAAACATAGCATTA-3′	This study	N/A
RT-PCR trio M Intron Retention (2) Reverse: 5′-ATCCGCTCGTTGAGAAACT-3′	This study	N/A
RT-PCR trio M Properly Spliced Forward: 5′-CGCTAAAGAGGAGCGCAATA-3′	This study	N/A
RT-PCR trio M Properly Spliced Reverse: 5′-CTTCTTAACACTCTTCATGATTCG-3′	This study	N/A
qRT-PCR trio M Intron Retention Forward: 5′-TTGAGTGAACCCGCTAAAG-3′	This study	N/A
qRT-PCR trio M Intron Retention Reverse: 5′-CTTTGGAGTGCTTGTTCTTATC-3′	This study	N/A
qRT-PCR trio M Properly Spliced Forward: CGTCCATAAATTGAGTCGGAGAAC	This study	N/A
qRT-PCR trio M Properly Spliced Reverse: 5′-CTTCTTAACACTCTTCATGATTCG-3′	This study	N/A
RT-PCR and qRT-PCR rpl32 Forward: AAGATGACCATCCGCCCAGCATAC	This study	N/A
RT-PCR and qRT-PCR rpl32 Reverse: ACGCACTCTGTTGTCGATACCCTT	This study	N/A
Software and algorithms
Fiji/ImageJ	(Schindelin et al., 2012; Rueden et al., 2017)	https://imagej.net/
Integrated Genome Viewer (IGV)	(Robinson et al., 2011)	https://igv.org/
Image Lab	Bio-Rad	https://image-lab-4-0.software.informer.com/
GraphPad (Prism)
Other
ChemiDoc	Bio-Rad	Catalogue no. 12003153
Humidified Incubators	Shel Lab	SRI20PF
#5 Dumont Fine Forceps	Ted Pella Inc.	Prod#5622
Motorized Pestle	Argos Technologies	Catalogue no. A0001
NanodropOne	Thermo Fisher Scientific	N/A