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Restriction enzymes required during the 4 different cloning steps in GoldenGate digestion-ligation reactions
| Cloning step | Restriction enzymes in Golden Gate cloning reaction |
|---|---|
| Oligo pairs into level 1 guide accepters | BsaI |
| Level 1 to level 2 | BsaI & BpiI |
| Level 1 to level M | BpiI |
| Level M to level 2 | BsaI & BpiI |
