FIG. 4.

Photograph of immunoblots of electrophoretically separated proteins from Vero cells and RSC infected with HSV-1(F), R7041 (U_S3⁻), R7356 (U_L13⁻), R7353 (U_S3⁻/U_L13⁻), and recombinant virus R7855. Replicate cultures of Vero cells (lanes 1 to 5) or RSC (lanes 6 to 10) were infected with the virus at an MOI of 5 and harvested at 18 h after infection. Proteins were solubilized in disruption buffer and electrophoretically separated in 11% denaturing polyacrylamide gels, transferred to nitrocellulose sheets, and reacted with a monoclonal antibody to U_S11 or polyclonal antibodies to ICP22 and U_L38 as described in Materials and Methods. All exposures for a given cell line were identical. Lane 4 was in a different position of the same gel. To maintain order in the presentation of data, the lane was cut and moved to its present location.