Figure 4.

P. gingivalis exerts its effect on iNKT cells through CHI3L1. A) CHI3L1 concentration in the culture supernatant of Pg-primed (+Pg) iNKT cells and unloaded moDC (CTRL). B) percentage of killed tumor cells by iNKT cells treated with increasing doses of rhCHI3L1. C) percentage of killed tumor cells by iNKT cells basal activated (CTRL), primed with 10⁹ CFUs of P. gingivalis (+Pg) or treated with rhCHI3L1 (100 ng/ml). D) Log₂ fold-change of the percentage of killed tumor cells by iNKT cells primed with 10⁹ CFUs of P. gingivalis (+Pg) or treated with rhCHI3L1 (100 ng/ml) upon neutralization with anti-CHI3L1 antibody (full bars); data have been normalized vs iNKT cells primed with unloaded moDC. E) western blot analysis; MFI is expressed as log₂ fold-change normalized vs iNKT cells primed with unloaded moDC. Data are representative of at least three independent experiments.