Fig. 7.

When producing a complete PCM containing both type-VI collagen and perlecan, a subset of ACPCs can produce type-II collagen earlier than ACs. A: Normalized MFI values of type-II collagen staining for cells that have produced both type-VI collagen and perlecan. This corresponds with the second quadrant as seen in Fig. 5D-F and H-J. Time points were compared with a Kruskal-Wallis test (ACs) or a repeated measures one-way ANOVA (ACPCs). Cell types were compared with a Mann-Whitney test (day 3) or an independent t-test (day 5, 10). Box and whisker plots show the median, first quartiles, and minimum and maximum values. ns: not significant. B-G: Histograms of modal type-II collagen intensity in Q2 showing variations between donors and individual cells. The colors represent individual donors (uniform across all figures).