Fig. 9.

Schematic overview of the gating strategy to analyze the flow cytometry data. A: Dot plot comparing droplets without (red) and with (blue) cells. The events of the cell-laden sample not overlapping with the events from the empty batch are considered cell-laden droplets. B: Histogram of DAPI staining of cell-laden microgels with gating to discriminate between live and dead cells. C: Type-VI collagen and perlecan staining of live cells at day 0. Quad gates are determined by assuming that there is no matrix present on day 0. The quad gates are copied to later time points of the same donor (D-F). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)