Figure 6.

Cross-linking of actin to DBP40 in transfected COS-1 cells. Cultured cells overexpressing DBP40 were treated with DSP and control cells incubated in parallel without DSP. Nuclear and cytoplasmic extracts were prepared from untreated (A) and treated (B) cells. Following urea treatment [lanes 2 and 4 in both (A) and (B)], the samples were subjected to T7-tag antibody agarose immunoprecipitation and bound proteins analyzed by SDS–PAGE and western blotting using an affinity-purified polyclonal antibody against actin and an HRP-conjugated anti-T7-tag monoclonal antibody to detect DBP40.