FIG. 5.

The 68-amino-acid Us11 RNA binding domain inhibits PKR activation. (A) Two micrograms of purified GST, GSTΔ1-87, or GSTΔ88-155 was subjected to electrophoresis on an SDS-polyacrylamide gel and stained with Coomassie blue. (B) Increasing amounts of either purified GST, GSTΔ1-87, or GSTΔ88-155 were added to 293 S10 extracts. Following the addition of reovirus dsRNA, the reaction mixtures were incubated at 30°C for 30 min. PKR was immunoprecipitated, and the immune complex was fractionated by SDS-PAGE. The gel was fixed and exposed to Kodak XAR film. Numbers at left of each panel indicate molecular masses in kilodaltons.