Skip to main content
. 2024 Jun 19;11(30):2307765. doi: 10.1002/advs.202307765

Figure 5.

Figure 5

CyI‐SS‐L decreased the mitochondrial membrane potential and induced cell apoptosis in KB‐C2 cells, KB‐3‐1 cells, MCF7 cells, or MCF7/taxol cells through the mitochondrial apoptosis pathway. A) The effect of CyI‐SS‐L on the mitochondrial membrane potential of KB‐C2 cells, KB‐3‐1 cells, MCF7 cells, and MCF7/taxol cells treated with CyI‐SS‐L (1 × 10−6 m) combined NIR radiation using JC‐1 probe (λ ex = 488 nm, λ em = 561 nm). Scale bar = 50 µm. B) The effect of CyI‐SS‐L (1 × 10−6 m) on cell apoptosis, analyzed by flow cytometry using membrane‐associated protein V‐FITC and PI in KB‐C2 cells, KB‐3‐1 cells, MCF7 cells, and MCF7/taxol cells under the NIR radiation. C) The bar graph presents the rate of cell apoptosis. D) The effect of CyI‐SS‐L on the expression of ABCB1, Caspase 9, and cleaved‐Caspase 9 proteins by Western blotting in MCF7/taxol cells and KB‐C2 cells. E) Use of densitometry to measure the intensity of the protein band quantified by AI600 images (*p < 0.5, **p < 0.01,***p < 0.001, **** p < 0.0001). Scale bars: 50 µm.