Figure 10.
Met@SBHA attenuates the aging process of cartilage in aged mice. A) SA‐β galactosidase staining of the cartilage layer in 3 M group, 17 M group, 17M+HAMA group, 17M+SBHA group, and 17M+Met@SBHA group. Scale bar 100 µm. B) Immunofluorescence staining to detect the expression of P21 in the cartilage layer in 3 M, 17 M, HAMA group, SBHA group, and Met@SBHA group. Scale bar 100 µm. C) Immunofluorescence staining of P53 in cartilage layers. Scale bar 100 µm. D–F) Quantitative analysis of the percentage of SA‐β‐gal‐positive chondrocytes, P21‐positive expressing cells and P53‐positive expressing cells in cartilage layer. Number of repetitions of all experiments n ≥ 3. Data are presented as the mean ± SD. (*P < 0.05; **P < 0.01; ***P < 0.001 and ****P <0.0001).