Figure 3.

Latest reported affinity‐based methods for EV preparation include: a) The mechanism for adhesion of EVs to a magnetic bead coated with choline phosphate, utilizing the high‐affinity interaction between phosphatidylcholine and choline phosphate, followed by subsequent release after capture. Reproduced with permission.^{[ 150 ]} Copyright 2023, American Association for the Advancement of Science. b) Isolation mechanism for structurally heterogeneous TCR‐CD3 EV subpopulations. Reproduced with permission.^{[ 152 ]} Copyright 2023, Wiley‐VCH, GmbH. c) The exosome isolation process using the SIMI system. Reproduced with permission.^{[ 153 ]} Copyright 2023, American Chemical Society. d) EVs captured via a bioorthogonal click chemistry reaction. Reproduced with permission.^{[ 155 ]} Copyright 2023, Wiley‐VCH, GmbH.