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. 2024 Jun 13;11(30):2309184. doi: 10.1002/advs.202309184

Figure 2.

Figure 2

Disrupted chromosomal synapsis in HFD fetal oocytes. a) Chromosome spreads of oocytes from E18.5 ND and HFD fetuses were immunostained for SYCP3 (green) and SYCP1 (red) at pachytene. Arrows indicate synapsed chromosomes, while arrowheads indicate a single chromosome. Magnified views of the synapsed region reveal that SYCP1 was localized in the central region of SCs in a continuous or discontinuous pattern in ND and HFD oocytes. b) The percentage of synapsed chromosome pairs in ND and HFD fetal oocytes. The percentages were determined by counting 180 ND cells across six biological replicates and 210 HFD cells across seven biological replicates. c) Chromosome spreads of oocytes from E18.5 ND and HFD fetuses were immunostained for SYCP3 (green) and CREST (purple) at pachytene. Arrowheads indicate CREST signals. d) CREST staining reveals unpaired chromosomes in HFD E18.5 female oocytes. CREST foci were counted in 180 ND and 168 HFD cells across 5 biological replicates. Data are presented as mean ± SD. Student's t‐test (two‐tailed) was employed for statistical analysis. Significance was set at P‐value <0.05.