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. 2024 Jun 13;11(30):2403516. doi: 10.1002/advs.202403516

Figure 5.

Figure 5

Lanifibranor moderately affects sphingolipid metabolism and release in vitro. A) HPLC‐ESI(+)‐HRMS was used to measure APAP metabolites on B) 2D hepatocyte culture and LoC. C) HPLC‐ESI‐MS/MS was used to measure FFA metabolites. Sphingolipids were measured on D) LoC perfusate, and E) conventional primary hepatocyte culture supernatant. Sample sizes: B,D,E) n = 4 per group. Abbreviations: APAP: acetaminophen; CYP: cytochrome P450; EIC: extracted ion chromatogram; F: free‐fatty acid treated; FL: free fatty acids plus lanifibranor treated; GST: glutathione S‐transferase; HPLC‐ESI‐HRMS: high‐performance liquid chromatography‐electrospray ionization high‐resolution mass spectrometry; HPLC‐ESI‐MS/MS: high‐performance liquid chromatography‐electrospray ionization tandem‐mass spectrometry; S1P: sphingosine‐1‐phosphate; V: vehicle. Paired student's t‐tests were performed. *p < 0.05 as indicated or as compared to vehicle.