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. 2024 Jun 6;11(30):2400700. doi: 10.1002/advs.202400700

Figure 4.

Figure 4

a) Biodistribution of 99mTc‐labeled Nbs 15 or 13, in Swiss nude (left), WT (middle) or uPAR KO (right) C57BL/6 mice bearing subcutaneous U87 or MC38 tumors, respectively. Micro‐SPECT/CT images were obtained 1 h after intravenous injection of 99mTc‐labeled Nbs. Arrows indicate Kd: kidneys; T: tumor; B: bladder; and Lu: lungs. Ex vivo biodistribution and tumor targeting of the b) anti‐HuPAR Nbs‐[99mTc]Tc and c) anti‐MuPAR Nbs‐[99mTc]Tc, together with radiolabeled non‐targeting control Nb. d) Comparison of Nb13‐[99mTc]Tc biodistribution between WT and uPAR KO C57BL/6 mice bearing a syngeneic MC38 tumor. Statistical analyses were performed using a Kruskal‐Wallis test followed by a Dunn's multiple comparisons test for the selection of the lead Nbs. A Mann‐Whitney test was used for comparison of Nb13‐[99mTc]Tc uptake between WT and uPAR KO mice. For all tests statistical significance was set at p < 0.05 (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).