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. Author manuscript; available in PMC: 2024 Aug 13.
Published in final edited form as: J Immunol. 2023 May 1;210(9):1272–1280. doi: 10.4049/jimmunol.2200605

Figure 2. IL-4 depletion does not influence somatic hypermutation or affinity maturation.

Figure 2.

The heavy chain B cell receptor sequence from hybridomas (n=7-8/group) generated by immunization with OXY-sKLH or OXY-sKLH with IL-4 depletion were analyzed against germline heavy chain sequences using IgBlast. A) Comparison of heavy chain CDR1 and CDR2 mutations in oxycodone-specific antibodies after OXY-sKLH immunization with or without IL-4 depletion. B) Sera from OXY-sKLH and OXY-sKLH+αIL-4 immunized mice from Figure 1 (n=6/group) were analyzed for avidity via biolayer interferometry. Data are mean±SEM. Statistical analysis performed via Mann Whitney U test. Data are from one independent experiment.